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Anti-IL15 Antibody LS-C104325

Note: This antibody replaces LS-C8165, LS-C54091, LS-C42719
Catalog Size Price
LS-C104325-50 50 µg Unavailable
LS-C104325-100 100 µg Unavailable

Related Products

129 IL15 Antibodies

Most Popular IL15 Antibodies

Anti-IL15 Antibody (Biotin) LS-C104326
Rabbit Polyclonal to Human IL15
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Biotin Conjugated
Immunohistochemistry Image
Anti-IL15 Antibody LS-C104510
Rabbit Polyclonal to Mouse IL15
Mouse
Western blot, ELISA, Neutralization
Unconjugated
Western blot Image
Anti-IL15 Antibody (clone AIO.3) IHC-plus™ LS-B9917
Rat Monoclonal [clone AIO.3] (IgG2a) to Mouse IL15
Mouse, Human
IHC - Paraffin, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-IL15 Antibody (Internal) IHC-plus™ LS-B10080
Rabbit Polyclonal (IgG) to Human IL15
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL15 Antibody (HRP) LS-C316680
Rabbit Polyclonal (IgG) to Human IL15
Human
ELISA
HRP Conjugated

100% Guaranteed
Rabbit Polyclonal to Human IL15
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human IL15
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.15 - 0.2 µg/ml)
  • Sandwich ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

IL15 antibody was raised against e.coli derived recombinant Human IL-15.
Human IL-15
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-15 (1 ng/ml), a concentration of 0.15-0.20 ug/ml of this antibody is required. ELISA: To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-15 (LS-C104326) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL15

P40933 NM_000585 NP_000576.1

IL15 Antibody, Interleukin-15 Antibody, IL-15 Antibody, Interleukin 15 Antibody

IL15 is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2.

Immunohistochemistry

Immunohistochemistry
IHC of IL-15 antibody LS-C104325. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of IL-15 antibody LS-C104325. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of IL-15 antibody LS-C104325. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of IL-15 antibody LS-C104325. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-15 antibody LS-C104325. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 6/23/2017

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