(tested or 100% immunogen sequence identity)
- Western blot
- Flow Cytometry
- Sandwich ELISA
IL13 antibody was raised against cOS-expressed recombinant human IL-13.
Recognizes human Interleukin-13 (IL-13).
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, No preservatives added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.