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Anti-IL13 Antibody (Azide-free) LS-C8144

Ordering

Wt. Vol. Conc. Price
500 µg - - $495
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rat Monoclonal (IgG1,k) to Human IL13
Human
Immunofluorescence, Western blot, Flow Cytometry, ELISA, Neutralization, Sandwich ELISA
Unconjugated, Azide-free

Details

Human IL13
Rat
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Purified
Azide-free

Applications

Immunofluorescence, Western blot, Flow Cytometry, ELISA, Neutralization, Sandwich ELISA

Specificity and Use

IL13 antibody was raised against cOS-expressed recombinant human IL-13.
Recognizes human Interleukin-13 (IL-13).
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.

Packaging

PBS. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About IL13

P35225 NM_002188 NP_002179.2

IL13 Antibody, ALRH Antibody, BHR1 Antibody, Interleukin-13 Antibody, NC30 Antibody, p600 Antibody, IL-13 Antibody, Interleukin 13 Antibody

IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines.

Requested From: United States
Date Requested: 9/25/2016

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