Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (0.5 - 1 µg/ml)
ELISA (2 - 4 µg/ml)
Specificity and Use
IL12 antibody was raised against cHO derived recombinant Human IL-12.
Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10 ug/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-12 when used with biotinylated antigen affinity purified anti-human IL-12 (LS-C104425) as the detection antibody at a concentration of approximately 4-8 ug/ml. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1 ng/lane, under non-reducing conditions.
Lyophilized from PBS
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.