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Anti-IL12 Antibody LS-C104440

Ordering

Wt. Vol. Conc. Price
500 µg - - $255
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL12 Antibodies

Anti-IL12 Antibody (Biotin) LS-C104425
Goat Polyclonal to Human IL12
Human
Western blot, ELISA, Neutralization
Biotin Conjugated
Western blot Image
Anti-IL12 Antibody LS-C104421
Goat Polyclonal to Mouse IL12
Mouse
Western blot, ELISA, Neutralization
Unconjugated
Enzyme-Linked Immunosorbent Assay Image
Anti-IL12 Antibody (Biotin) LS-C24868
Mouse Monoclonal (IgG1,k) to Rat IL12
Rat
ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human IL12
Human
IHC, Western blot, ELISA
Unconjugated

Details

Human IL12
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • Western blot (0.5 - 1 µg/ml)
  • ELISA (2 - 4 µg/ml)

Specificity and Use

IL12 antibody was raised against cHO derived recombinant Human IL-12.
Human IL-12
Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10 ug/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-12 when used with biotinylated antigen affinity purified anti-human IL-12 (LS-C104425) as the detection antibody at a concentration of approximately 4-8 ug/ml. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1 ng/lane, under non-reducing conditions.

Packaging

Lyophilized from PBS
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL12


Immunohistochemistry

Immunohistochemistry
IHC of IL12 antibody LS-C104440
 

Requested From: United States
Date Requested: 12/9/2016

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