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Catalog Number Size Price
LS-C154115-100 100 µg (1 mg/ml) $552 
IL-33 Antibody - Western blot using anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti‑Human IL‑33 Antibody (Biotin, IHC, WB) LS‑C154115

Polyclonal Rabbit anti‑Human IL‑33 Antibody (Biotin, IHC, WB) LS‑C154115

Antibody:
IL-33 Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Biotin, Unmodified
Price
Catalog Number
$552
LS-C154115-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL-33 Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Biotin, Unmodified

Specifications

Description
IL-33 antibody LS-C154115 is a biotin-conjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA, IHC and WB.
Target
Human IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Antiserum
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Specificity
This antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 18 kD in size corresponding to mature human IL-32a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-33
O95760 NM_033439 NP_254274.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024