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Anti-IL-1B / IL-1 Beta Antibody (N-Terminus) LS-C18827

Note: This antibody replaces LS-C7719, LS-C70852

Ordering

Wt. Vol. Conc. Price
1000 µg - 2 mg/ml $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL-1B / IL-1 Beta Antibodies

Anti-IL-1B / IL-1 Beta Antibody (clone 3E1) LS-C174781
Mouse Monoclonal [clone 3E1] (IgG1) to Human IL-1B / IL-1 Beta
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IL-1B / IL-1 Beta Antibody (clone 3A6) LS-C169431
Mouse Monoclonal [clone 3A6] (IgG1) to Human IL-1B / IL-1 Beta
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL-1B / IL-1 Beta Antibody (clone AS10) LS-C26495
Mouse Monoclonal [clone AS10] (IgG1)
Human
IHC, Immunoprecipitation, ELISA, Neutralization
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human IL-1B / IL-1 Beta
Human, Dog, Primate
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Unconjugated

Details

Human IL-1B / IL-1 Beta
Rabbit
Human, Dog, Primate (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Purified IgG
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:200)
  • IHC - Frozen (1:100 - 1:200)
  • Western blot (1:100 - 1:200)
  • Immunoprecipitation (1:400 - 1:800)
  • ELISA (1:500 - 1:2000)
  • Radioimmunoassay (1:5000 - 1:10000)

Specificity and Use

IL-1B / IL-1 Beta antibody was raised against recombinant human IL1B / IL1 Beta.
N-Terminus
Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (2)

1
Serum soluble interleukin 7 receptor is strongly associated with lupus nephritis in patients with systemic lupus erythematosus. Badot V, Luijten RK, van Roon JA, Depresseux G, Aydin S, Van den Eynde BJ, Houssiau FA, Lauwerys BR. Annals of the rheumatic diseases. 2013 72:453-6. (IHC-Fr; Human) [PubMed:23264357]
2
p65-Dependent production of interleukin-1 beta by osteolytic prostate cancer cells causes an induction of chemokine expression in osteoblasts. Schulze J, Weber K, Baranowsky A, Streichert T, Lange T, Spiro AS, Albers J, Seitz S, Zustin J, Amling M, Fehse B, Schinke T. Cancer letters. 2012 Apr;317:106-13. [Full Text Article] [PubMed:22108531]

About IL-1B / IL-1 Beta

P01584 NM_000576 NP_000567.1

IL1B Antibody, IL-1B Antibody, IL-1 beta Antibody, IL1-BETA Antibody, Preinterleukin 1 beta Antibody, Pro-interleukin-1-beta Antibody, Catabolin Antibody, IL-1 Antibody, IL1F2 Antibody, Interleukin 1, beta Antibody, Interleukin-1 beta Antibody

IL-1 Beta is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.

Western blot

Western blot
Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/11/2016

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