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Catalog Number Size Price
LS-C154088-100 100 µg (1 mg/ml) $567 
IL-1B / IL-1 Beta Antibody - Anti-Human IL-1beta Antibody - Western Blot. This antibody is primarily directed against mature, 17000 MW human IL-1beta and is useful in determining its presence in various assays.

Polyclonal Rabbit anti‑Human IL‑1B / IL‑1 Beta Antibody (N‑Terminus, WB) LS‑C154088

Polyclonal Rabbit anti‑Human IL‑1B / IL‑1 Beta Antibody (N‑Terminus, WB) LS‑C154088

Antibody:
IL-1B / IL-1 Beta Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
WB
Reactivity:
Human, Dog, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154088-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL-1B / IL-1 Beta Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
WB
Reactivity:
Human, Dog, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
IL-1 Beta antibody LS-C154088 is an unconjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) (N-Terminus) from human. It is reactive with human, dog and primate. Validated for WB.
Target
Human IL-1B / IL-1 Beta
Synonyms
IL1B | IL-1B | IL-1 beta | IL1-BETA | Preinterleukin 1 beta | Pro-interleukin-1-beta | Catabolin | IL-1 | IL1F2 | Interleukin 1, beta | Interleukin-1 beta
Host
Rabbit
Reactivity
Human, Dog, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
DEAE fractionation
Modifications
Unmodified
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli.  The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117.  This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1).
Epitope
N-Terminus
Specificity
This antibody is primarily directed against mature, 17,000 MW human IL-1ß.
Applications
  • Western blot (1:500 - 1:2000)
Usage
Anti-Human IL-1beta has been tested for use in immunoblotting. It recognizes the 17000 MW mature IL-1beta. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E. coli LPS.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-1B / IL-1 Beta
P01584 NM_000576 NP_000567.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024