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Anti-IL-1B / IL-1 Beta Antibody (HRP) LS-C154137

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL-1B / IL-1 Beta Antibodies

Anti-IL-1B / IL-1 Beta Antibody (clone AS10) LS-C26495
Mouse Monoclonal [clone AS10] (IgG1)
Human
IHC, Immunoprecipitation, ELISA, Neutralization
Unconjugated
Anti-IL-1B / IL-1 Beta Antibody (clone 3A6) LS-C169431
Mouse Monoclonal [clone 3A6] (IgG1) to Human IL-1B / IL-1 Beta
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL-1B / IL-1 Beta Antibody (clone 3E1) LS-C174781
Mouse Monoclonal [clone 3E1] (IgG1) to Human IL-1B / IL-1 Beta
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse IL-1B / IL-1 Beta
Mouse, Human, Rat
IHC, Western blot, Flow Cytometry, ELISA
HRP Conjugated

Details

Mouse IL-1B / IL-1 Beta
Rabbit
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Horseradish Peroxidase
Purified, heated to 56°C for 30 minutes
Unmodified

Applications

  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • Flow Cytometry
  • ELISA

Specificity and Use

IL-1B / IL-1 Beta antibody was raised against this antibody was prepared by repeated immunizations with recombinant mouse IL-1 produced in E. coli. The MW of recombinant mouse IL-1 was 17 kD.
This antibody was heated to 56° C for 30 minutes. This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17000 MW mouse IL-1beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1beta. This antibody will recognize 10% of the non-denatured (native) precursor 31000 MW mouse IL-1beta containing samples but will primarily detect all of the 17000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31000 MW IL- 1beta precursor molecule. Denatured 31000 precursor IL-1beta will be recognized by this antibody.
Anti-Mouse IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-1beta. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E. coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1beta. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1beta activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of human or primate IL-1beta. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL-1B / IL-1 Beta

P01584 NM_000576 NP_000567.1

IL1B Antibody, IL-1B Antibody, IL-1 beta Antibody, IL1-BETA Antibody, Preinterleukin 1 beta Antibody, Pro-interleukin-1-beta Antibody, Catabolin Antibody, IL-1 Antibody, IL1F2 Antibody, Interleukin 1, beta Antibody, Interleukin-1 beta Antibody

IL-1 Beta is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.

Western blot

Western blot
IL1 beta Antibody Peroxidase Conjugated Western Blot. Western Blot of Rabbit anti-Mouse IL-1 Beta Antibody Peroxidase Conjugated Lane 1: Mouse IL-1 Beta Load: 50 ng per lane Secondary antibody: IL1 beta Antibody Peroxidase Conjugated at 1:1000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 18 kD, 18 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/6/2016

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