Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3E1] (IgG1) to Human IL-1B / IL-1 Beta
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Mouse Monoclonal [clone AS56] (IgG1) to Human IL-1B / IL-1 Beta
Human IL-1B / IL-1 Beta
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [AS56]
Specificity and Use
Human IL1B / IL1 Beta
Applications: ELISA: This antibody has been used as a solution phase antibody at 2 ug/ml in a two site ELISA with biotin conjugated IL-1beta antibody (Cat. No. L142M). Typically the ELISA has a working range of 0-500 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 l. Recovery of hIL-1 (in human serum is typically >85% in the ELISA.
IL-1 Beta is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.