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Anti-IL-10 Antibody LS-C59384


Wt. Vol. Conc. Price
- 1000 µl - $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IL-10 Antibodies

Anti-IL-10 Antibody (clone JES5-2A5) IHC-plus™ LS-B4913
Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL-10
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IL-10
Western blot, ELISA


Human IL-10
Human (tested or 100% immunogen sequence identity)


  • Western blot

Specificity and Use

IL-10 antibody was raised against recombinant human IL-10 produced in E. coli.
IL-10 present in body fluids and cell supernatants in various assays (i. e. IL-1 stimulated IL10 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10.
This antiserum has been tested for use in ELISA and Western blot.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About IL-10

P22301 NM_000572 NP_000563.1

IL10 Antibody, CSIF Antibody, GVHDS Antibody, IL-10 Antibody, IL10A Antibody, Interleukin-10 Antibody, Interleukin 10 Antibody, TGIF Antibody

IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Western blot

Western blot
Anti-IL-10 Antibody. Anti-IL-10 whole antiserum (LS-C59384) was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4°C and probed with LS-C59384 diluted 1:200 in 3% BSA-TBS o/n 4°C. Primary antibody was detected with Gt anti-Rb LS-C60865 Lot#21231 1:20000 in MB-070 1 Hour 4°C using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Bio-Rad. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/27/2016

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