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Anti-IL-10 Antibody (Azide-free) LS-C8121

Ordering

Wt. Vol. Conc. Price
500 µg - - $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rat Monoclonal (IgG1,k) to Human IL-10
Human
Immunofluorescence, Western blot, Flow Cytometry, ELISA, Neutralization
Unconjugated, Azide-free

Details

Human IL-10
Rat
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Purified
Azide-free

Applications

Immunofluorescence, Western blot, Flow Cytometry, ELISA, Neutralization

Specificity and Use

IL-10 antibody was raised against cOS-expressed human IL-10.
Recognizes human and viral Interleukin-10 (IL-10).
ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified antibody should be paired with biotinylated monoclonal antibody as the detection antibody, with purified recombinant human IL-10 as the standard. Immunofluorescence/. Flow Cytometry: The antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-10 under reducing conditions. Neutralization: Can be used for neutralizing human/viral IL-10 bioactivity. A suitable Rat IgG1 should be used as an isotype control.

Packaging

PBS. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About IL-10

P22301 NM_000572 NP_000563.1

IL10 Antibody, CSIF Antibody, GVHDS Antibody, IL-10 Antibody, IL10A Antibody, Interleukin-10 Antibody, Interleukin 10 Antibody, TGIF Antibody

IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Requested From: United States
Date Requested: 9/26/2016

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