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Anti-IL-10 Antibody (Azide-free) LS-C8121

Ordering

Wt. Vol. Conc. Price
500 µg - - $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL-10 Antibodies

Anti-IL-10 Antibody (clone JES5-2A5) IHC-plus™ LS-B4913
Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL-10
Mouse
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG1,k) to Human IL-10
Human
Immunofluorescence, Western blot, Flow Cytometry, ELISA, Neutralization
Unconjugated, Azide-free

Details

Human IL-10
Rat
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Purified
Azide-free

Applications

  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
  • Neutralization

Specificity and Use

IL-10 antibody was raised against cOS-expressed human IL-10.
Recognizes human and viral Interleukin-10 (IL-10).
ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified antibody should be paired with biotinylated monoclonal antibody as the detection antibody, with purified recombinant human IL-10 as the standard. Immunofluorescence/. Flow Cytometry: The antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-10 under reducing conditions. Neutralization: Can be used for neutralizing human/viral IL-10 bioactivity. A suitable Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Packaging

PBS. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About IL-10

P22301 NM_000572 NP_000563.1

IL10 Antibody, CSIF Antibody, GVHDS Antibody, IL-10 Antibody, IL10A Antibody, Interleukin-10 Antibody, Interleukin 10 Antibody, TGIF Antibody

IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Requested From: United States
Date Requested: 12/4/2016

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