Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Goat Polyclonal (IgG) to Human IKBKE / IKKI / IKKE
ICC, Western blot
Human IKBKE / IKKI / IKKE
Human (tested or 100% immunogen sequence identity)
ICC (1.7 - 15 µg/ml)
Western blot (1 µg/ml)
Specificity and Use
IKBKE / IKKI / IKKE antibody was raised against e. coli-derived rhIKKepsilon.
Human IKK epsilon.
Western blot-An antibody concentration of 1.0 µg/ml is recommended. Immunocytochemistry-This antibody was used at a concentration of 1.7-15 µg/ml with appropriate secondary reagents to detect IKK epsilon in human peripheral blood mononuclear cells.
Lyophilized from 0.2 um filtered solution in PBS, 5% trehalose
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X.
Detection of IKK epsilon. Lysates of human Raji, MDA-MB-468, and Ramos cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 ug/ml anti-IKK epsilon. A three minute exposure to film is shown.