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| Catalog Number | Size | Price |
|---|---|---|
| LS-B715-50 | 50 µl (80 mg/ml) | $515 |
![IKBKB / IKK2 / IKK Beta Antibody - Anti-IKKbeta Antibody - Western Blot. Western blot of anti-IKKbeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1h. Washes with TBS preceded color development.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-ikbkb-ikk2-ikk-beta-antibody-c-terminus-ls-b715/62514_118744.jpg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human IKBKB / IKK2 / IKK Beta Antibody (C‑Terminus, IHC, WB) LS‑B715
IHC‑plus™ Polyclonal Rabbit anti‑Human IKBKB / IKK2 / IKK Beta Antibody (C‑Terminus, IHC, WB) LS‑B715
Note: This antibody replaces LS-C18941
Antibody:
IKBKB / IKK2 / IKK Beta Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IKBKB / IKK2 / IKK Beta Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
IKK Beta antibody LS-B715 is an unconjugated rabbit polyclonal antibody to IKK Beta (IKBKB / IKK2) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IKBKB / IKK2 / IKK Beta
Synonyms
IKBKB | Ikk 2 | IKK-B | IKK2 | IKKbeta | NFKBIKB | I-kappa-B kinase 2 | IKKB | I-kappa-B-kinase beta | Ikb kinase-beta | IKK Beta | IKK-beta
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
IKKb peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
C-Terminus
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:200 - 1:1000)
- ELISA (1:1000 - 1:5000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B715 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B715 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IKBKB / IKK2 / IKK Beta
Validation
Anti-IKBKB / IKK Beta antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-IKBKB / IKK Beta antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ IKBKB / IKK2 / IKK Beta Antibody (C-Terminus) for IHC, WB/Western, ELISA LS-B715 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Western blot
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/22/2024
Date Requested: 4/22/2024
