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Anti-IGFBP5 Antibody IHC-plus™ LS-B8207

Note: This antibody replaces LS-C104306, LS-C41982

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IGFBP5 Antibodies

Anti-IGFBP5 Antibody IHC-plus™ LS-B6938
Rabbit Polyclonal (IgG) to Human IGFBP5
Human, Monkey, Bat, Bovine, Dog, Goat, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IGFBP5 Antibody IHC-plus™ LS-B8207
Rabbit Polyclonal to Human IGFBP5
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IGFBP5
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human IGFBP5
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)

Specificity and Use

IGFBP5 antibody was raised against e.coli derived recombinant Human IGF-BP5.
Human IGF-BP5
Immunohistochemistry: LS-B8207 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hIGF-BP5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP5 (LS-C104305) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IGFBP5

P24593 NM_000599 NP_000590.1

IGFBP5 Antibody, IBP-5 Antibody, IGFBP-5 Antibody, IBP5 Antibody, IGF-binding protein 5 Antibody

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Immunohistochemistry

Immunohistochemistry
Anti-IGFBP-5 antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8207 dilution 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of IGFBP-5 antibody LS-B8207

Western blot

Western blot
Western Blot (reducing) of IGFBP-5 antibody LS-B8207

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IGFBP-5 antibody LS-B8207

Requested From: United States
Date Requested: 12/10/2016

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