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Anti-IGFBP3 Antibody IHC-plus™ LS-B7473

Note: This antibody replaces LS-C104487


Wt. Vol. Conc. Price
50 µg 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IGFBP3 Antibodies

Anti-IGFBP3 Antibody (clone 83.8F9) LS-C45037
Mouse Monoclonal [clone 83.8F9] (IgG1) to Human IGFBP3
ELISA, Radioimmunoassay
Anti-IGFBP3 Antibody (aa191-205) LS-C61755
Goat Polyclonal to Human IGFBP3
Human, Mouse, Rat, Bovine, Dog, Goat, Horse, Pig, Sheep
Western blot, ELISA
Western blot Image
Anti-IGFBP3 Antibody LS-C149975
Goat Polyclonal (IgG) to Human IGFBP3
IHC - Paraffin, Western blot, Neutralization
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IGFBP3
IHC - Paraffin, Western blot, ELISA


Human IGFBP3
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)

Specificity and Use

IGFBP3 antibody was raised against e.coli derived recombinant Human IGF-BP3.
Human IGF-BP3
Immunohistochemistry: LS-B7473 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hIGF-BP3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP3 (LS-C104468) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP3. Western Blot: To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.


Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IGFBP3

P17936 NM_000598 NP_000589.2

IGFBP3 Antibody, Binding protein 29 Antibody, Binding protein 53 Antibody, BP-53 Antibody, IGF-binding protein 3 Antibody, IGFBP-3 Antibody, IBP-3 Antibody, IBP3 Antibody

IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.


Anti-IGFBP3 antibody IHC of human liver, hephatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7473 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of IGFBP3 antibody LS-B7473

Western blot

Western blot
Western Blot (reducing) of IGFBP3 antibody LS-B7473

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IGFBP3 antibody LS-B7473

Requested From: United States
Date Requested: 10/20/2016

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