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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IGFBP2 / IGF‑BP53 Antibody (aa182‑231) LS‑C81847
IGF-BP53 antibody LS-C81847 is an unconjugated rabbit polyclonal antibody to IGF-BP53 (IGFBP2) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C81847-50
50 µg
Unavailable

Popular IGFBP2 / IGF-BP53 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Bovine, Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation, ELISA
IHC of paraffin-embedded human lung cancer (A), recturn(B), prostate (C), colon cancer (D) showing cytoplasmic localization using IGFBP2 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, Western blot, ELISA
Anti-IGFBP2 / IGF-BP53 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot

Product Description

IGF-BP53 antibody LS-C81847 is an unconjugated rabbit polyclonal antibody to IGF-BP53 (IGFBP2) from human, mouse, rat and other species. Validated for WB.
About IGFBP2 / IGF-BP53
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. P18065 NM_000597 NP_000588.2

IGFBP2 Antibody, BP2 Antibody, IGFBP-2 Antibody, IGF-binding protein 2 Antibody, IBP-2 Antibody, IBP2 Antibody, IGF-BP53 Antibody


Specifications

Target
Human IGFBP2 / IGF-BP53
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Goat, Guinea pig, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Predicted
Monkey, Dog, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa182-231 of human IGFBP2 (P18065, NP_000588). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Sheep, Goat, Elephant, Bovine, Pig, Guinea pig (100%); Marmoset, Dog, Horse (92%).
Blocking Peptide
LS-E19549 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81847. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa182-231
Specificity
Human IGFBP2
Usage
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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