Human IGF1R / IGF1 Receptor
(tested or 100% immunogen sequence identity)
Also available conjugated with RPE
Protein G purified
Also available Low Endotoxin
- IHC - Paraffin (10 µg/ml)
- Western blot
- Flow Cytometry (1:10)
- (applications tested for the base form of this product only)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221.
Immunohistochemistry: LS-B2613 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2613 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Histology Positive Control Tissue: Pancreas, placenta.
PBS, pH 7.4, 0.09% sodium azide.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.