Human IGF1R / IGF1 Receptor
Human, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
It weakly mimics IGF-1 action. Reacts only weakly with hybrids of Insulin Receptor and IGF-1R while it reacts very well.3 So, it has proved useful in the isolation of hybrid receptors.3 Species cross-reactivity: human and rabbit. Weakly reacts with porcine. Does not react with rat.
Affinity Purification of Hybrid Receptors3. Acts as a Weak IGF-like Agonist in Bioassays1 (Thymidine incorporation into DNA). Inhibits (>90%) IGF-1 Binding1 (Ab 100nM) (=1.5 ug/ml). Immunoprecipitation (Not optimum). Western Blot (Not suitable). Positive control: Placenta or breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
10 mM PBS, pH 7.4
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.