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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IGF1R / IGF1 Receptor Antibody (aa1264‑1367, clone JBW902) IHC‑plus™ LS‑B2905
Note: This antibody replaces LS-C7533
IGF1 Receptor antibody LS-B2905 is an unconjugated mouse monoclonal antibody to IGF1 Receptor (IGF1R) from human and mouse. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2905-50
50 µg (1 mg/ml)
Unavailable

Popular IGF1R / IGF1 Receptor Products

IHC of paraffin-embedded human prostate carcinoma using LS-C24709.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
IHC of paraffin-embedded human stomach mucosae showing membrane location using IGF1R antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-IGF1 Receptor antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2613 concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human, Rabbit
Applications: Immunoprecipitation, ELISA

Product Description

IGF1 Receptor antibody LS-B2905 is an unconjugated mouse monoclonal antibody to IGF1 Receptor (IGF1R) from human and mouse. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About IGF1R / IGF1 Receptor
Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. P08069 NM_000875 NP_000866.1

IGF1R Antibody, CD221 antigen Antibody, CD221 Antibody, IGF1 Receptor Antibody, IGFR Antibody, IGF-I receptor Antibody, Soluble IGF1R variant 1 Antibody, Soluble IGF1R variant 2 Antibody, Type 1 IGF receptor Antibody, IGFIR Antibody, JTK13 Antibody


Specifications

Target
Human IGF1R / IGF1 Receptor
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [JBW902]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor.
Epitope
aa1264-1367
Specificity
Recognizes human IGF-1 receptor beta chain, MW 95kD. Recognizes transmembrane domain. Not expected to cross-react with INSR based on sequence homology.
Usage
Immunohistochemistry: LS-B2905 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2905 was determined to be 5 ug/ml. Western Blot: 0.1-2 ug/ml. Detects the beta chain of IGF1R in RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. C4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2905. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug/ml immunoprecipitates IGF1R from 1 mg of RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Immunohistochemistry

Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.
Anti-IGF1 Receptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2905 concentration 5 ug/ml.

Requested From: 
Date Requested: 7/17/2018

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