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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2293-50 | 50 µg (0.5 mg/ml) | $485 |
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IHC‑plus™ Polyclonal Goat anti‑Human IFT74 / CCDC2 Antibody (aa588‑600, IHC, WB) LS‑B2293
IHC‑plus™ Polyclonal Goat anti‑Human IFT74 / CCDC2 Antibody (aa588‑600, IHC, WB) LS‑B2293
Note: This antibody replaces LS-C54662, LS-C79456
Antibody:
IFT74 / CCDC2 Goat anti-Human Polyclonal (aa588-600) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IFT74 / CCDC2 Goat anti-Human Polyclonal (aa588-600) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
CCDC2 antibody LS-B2293 is an unconjugated goat polyclonal antibody to CCDC2 (IFT74) (aa588-600) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Host
Goat
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KTIVDALHSTSGN, from the C-Terminus of protein sequence according to NP_001092692.1NP_001092693.1NP_079379.2AAK77221.1.
Epitope
aa588-600
Specificity
Human IFT74.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 0.6 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2293 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2293 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IFT74 / CCDC2
Validation
Anti-IFT74 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IFT74 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ IFT74 / CCDC2 Antibody (aa588-600) for IHC, WB/Western LS-B2293 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. Momeni P, Schymick J, Jain S, Cookson MR, Cairns NJ, Greggio E, Greenway MJ, Berger S, Pickering-Brown S, Chi A, Fung HC, Holtzman DM, Huey ED, Wassermann EM, Adamson J, Hutton ML, Rogaeva E, St George-Hyslop P, Rothstein JD, Hardiman O, Grafman J, Single. BMC neurology. 2006 6:44.
Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice. Boldt K, Mans DA, Won J, van Reeuwijk J, Vogt A, Kinkl N, Letteboer SJ, Hicks WL, Hurd RE, Naggert JK, Texier Y, den Hollander AI, Koenekoop RK, Bennett J, Cremers FP, Gloeckner CJ, Nishina PM, Roepman R, Ueffing M. The Journal of clinical investigation. 2011 121:2169-80.
Customer Reviews (0)
Featured Products
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
