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Catalog Number Size Price
LS-B2107-0.05 0.05 mg (1 mg/ml) $545 
IFIH1 / MDA5 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.
IFIH1 / MDA5 Antibody - Western blot analysis of Anti-MDA5 antibody (LS-B2107, 0.4 µg/ml; 15 µg of lysate per lane). Lane 1: Daudi cell line. Lane 2: HeLa cell line. Antibody produced a predominant band at ~117kDA in both cell lines.
IFIH1 / MDA5 Antibody - Western blot analysis of Anti-MDA5 antibody in Daudi Cell lysate (LS-B2107). Lane 1: 1 µg/ml. Lane 2: 2 µl. Lane 3: 4 µg/ml. Antibody produced a predominant band at ~117kDA in Daudi cell line at all concentrations.
IFIH1 / MDA5 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IFIH1 / MDA5 Antibody - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.
IFIH1 / MDA5 Antibody - Western blot analysis of Anti-MDA5 antibody (LS-B2107, 0.4 µg/ml; 15 µg of lysate per lane). Lane 1: Daudi cell line. Lane 2: HeLa cell line. Antibody produced a predominant band at ~117kDA in both cell lines.
IFIH1 / MDA5 Antibody - Western blot analysis of Anti-MDA5 antibody in Daudi Cell lysate (LS-B2107). Lane 1: 1 µg/ml. Lane 2: 2 µl. Lane 3: 4 µg/ml. Antibody produced a predominant band at ~117kDA in Daudi cell line at all concentrations.
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IHC‑plus™ Polyclonal Rabbit anti‑Human IFIH1 / MDA5 Antibody (IHC, IF, WB) LS‑B2107

IHC‑plus™ Polyclonal Rabbit anti‑Human IFIH1 / MDA5 Antibody (IHC, IF, WB) LS‑B2107

Note: This antibody replaces LS-C19677
Antibody:
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$545
LS-B2107-0.05
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MDA5 antibody LS-B2107 is an unconjugated rabbit polyclonal antibody to MDA5 (IFIH1) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IFIH1 / MDA5
Synonyms
IFIH1 | Helicase with 2 CARD domains | MDA-5 | MDA5 | Helicard | IDDM19 | RLR-2 | RH116 | CADM-140 autoantigen | Hlcd | RIG-I-like receptor 2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
A 16 AA synthetic peptide near the C-Terminus of human MDA5. The immunogen is located within the last 50 AA of MDA5.
Applications
  • IHC (5 µg/ml)
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot (1 - 4 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 113 kDa Observed: 115 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IFIH1 / MDA5
Q9BYX4 NM_022168 NP_071451.2

Validation

IFIH1 / MDA5 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

IFIH1 / MDA5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

IFIH1 / MDA5 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

IFIH1 / MDA5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of mouse pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of mouse pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ IFIH1 / MDA5 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B2107 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024