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Anti-IER3 / IEX-1 Antibody (aa38-65) LS-C165117

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular IER3 / IEX-1 Antibodies

Anti-IER3 / IEX-1 Antibody (Internal) IHC-plus™ LS-B1360
Rabbit Polyclonal (IgG) to Human IER3 / IEX-1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IER3 / IEX-1 Antibody (aa38-65) LS-C165117
Rabbit Polyclonal to Human IER3 / IEX-1
Human, Mouse
IHC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-IER3 / IEX-1 Antibody (aa38-65, AP) LS-C259017
Rabbit Polyclonal (IgG) to Human IER3 / IEX-1
Human, Mouse
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IER3 / IEX-1
Human, Mouse
IHC, Western blot, Flow Cytometry
Unconjugated

Details

Human IER3 / IEX-1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E1221 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C165117. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa38-65
This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human IER3.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About IER3 / IEX-1

P46695 NM_003897 NP_003888.2

IER3 Antibody, DIF-2 Antibody, DIF2 Antibody, Gly96, mouse, homolog of Antibody, IEX1 Antibody, IEX-1L Antibody, Immediate early protein GLY96 Antibody, Immediate early response 3 Antibody, Immediately early gene X-1 Antibody, GLY96 Antibody, IEX-1 Antibody, Protein DIF-2 Antibody, Protein PRG1 Antibody, PRG1 Antibody, Anti-death protein Antibody

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.

Immunohistochemistry

Immunohistochemistry
IER3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of IER3 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
IER3 Antibody western blot of mouse heart tissue lysates (35 ug/lane). The IER3 antibody detected the IER3 protein (arrow).

Western blot

Western blot
IER3 Antibody western blot of A549 cell line lysates (35 ug/lane). The IER3 antibody detected the IER3 protein (arrow).

Flow Cytometry

Flow Cytometry
IER3 Antibody flow cytometry of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: 
Date Requested: 3/25/2017

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