Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone OTI2G4] (IgG1) to Human IDO1 / IDO
IHC - Paraffin, Immunofluorescence, Western blot
Rabbit Polyclonal (IgG) to Rat IDO1 / IDO
IHC - Paraffin, IHC - Frozen, Western blot
Rat IDO1 / IDO
Rat, Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Specificity and Use
IDO1 / IDO antibody was raised against a mixture of three rat IDO peptides: MPHSQISPAEGSRRILEEY (1-19), LFSFPGGDCDKGFFLVSLMVE (155-176), and VKPSKQKPMGGHKSEEPS (359-376) coupled to keyhole limpet hemocyanin.
Rat IDO1 / IDO. Does not bind IDO2.
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For immunohistology antigen retrieval is necessary.
IDO1 / IDO is indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity.