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Anti-IDO1 / IDO Antibody (clone 2E2.6) LS-C153837

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IDO1 / IDO Antibodies

Anti-IDO1 / IDO Antibody (aa129-141) IHC-plus™ LS-B1746
Goat Polyclonal to Human IDO1 / IDO
Human, Monkey, Mouse
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IDO1 / IDO Antibody (clone 2E2.6) LS-C153837
Mouse Monoclonal [clone 2E2.6] (IgG1) to Mouse IDO1 / IDO
Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IDO1 / IDO Antibody (clone 2G4) LS-C174759
Mouse Monoclonal [clone 2G4] (IgG1) to Human IDO1 / IDO
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2E2.6] (IgG1) to Mouse IDO1 / IDO
Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

Details

Mouse IDO1 / IDO
Mouse
Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2E2.6]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:1500)
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA (1:5000 - 1:50000)

Specificity and Use

IDO1 / IDO antibody was raised against iDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.
IDO1 antibody is specific for mouse IDO1 protein. Mouse IDO1 does not react with human tissues. Cross-reactivity with IDO1 from other sources has not been determined.
Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IF, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IDO1 / IDO

P14902 NM_002164 AAA36081.1

IDO1 Antibody, IDO Antibody, Indole 2,3-dioxygenase Antibody, Indolamine 2,3 dioxygenase Antibody, IDO-1 Antibody, INDO Antibody, Indoleamine 2,3-dioxygenase Antibody, Indoleamine 2,3-dioxygenase 1 Antibody

IDO1 / IDO is indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity.

Immunohistochemistry

Immunohistochemistry
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunocytochemistry

Immunocytochemistry
Immunofluorescence Microscopy of Mouse Anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).

Western blot

Western blot
Western Blot of mouse anti-IDO1 antibody. Lane 1: HEK293 control vector. Lane 2: HEK293 expressing mouse IDO1. Lane 3: HEK293 expressing mouse IDO2. Load: 35 µg per lane. Primary antibody: IDO 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.6 kDa, ~44 kDa for IDO1. Other band(s): non-specifics.

Western blot

Western blot
Western Blot of Mouse Anti-IDO1 Antibody. Extracts from 293HEK Cells expressing: Lane 1: Control Vector. Lane 2: His-tagged mouse IDO1. Lane 3: mouse IDO1. Lane 4: His-tagged mouse IDO2. Lane 5: mouse IDO2. Lane 6: Epididymis from IDO null. Lane 7: wild type mice. Primary antibody: IDO-1(2E2) monoclonal antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBST overnight at 4°C. Predicted/Observed size: 41-42 kDa/41-42 kDa for IDO-1. Other band(s): none.

Flow Cytometry

Flow Cytometry
Flow Cytometry of Mouse Anti-IDO1 antibody. Cells: HEK293 cells. Expresing: mouse IDO-1(blue) and mouse IDO-2 (red). Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.
 

Requested From: United States
Date Requested: 12/3/2016

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