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Anti-IDO1 / IDO Antibody (clone 1E11) LS-C174765

Catalog Size Price
LS-C174765-100 100 µl (1 mg/ml) Unavailable

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100 IDO1 / IDO Antibodies

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Western blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells. Lane 2: IFN-r treated HeLa cells. Load: 35 ug per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.
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100% Guaranteed
Mouse Monoclonal [clone 1E11] (IgG1) to Human IDO1 / IDO
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human IDO1 / IDO
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1E11]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:4000)

Specificity and Use

IDO1 / IDO antibody was raised against full length human recombinant protein of human IDO1(NP_002155) produced in HEk293T cell.
Human IDO

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About IDO1 / IDO

P14902 NM_002164 AAA36081.1

IDO1 Antibody, IDO Antibody, Indole 2,3-dioxygenase Antibody, Indolamine 2,3 dioxygenase Antibody, IDO-1 Antibody, INDO Antibody, Indoleamine 2,3-dioxygenase Antibody, Indoleamine 2,3-dioxygenase 1 Antibody

IDO1 / IDO is indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IDO1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IDO1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-IDO1 mouse monoclonal antibody.
IHC of paraffin-embedded Human liver tissue using anti-IDO1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-IDO1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-IDO1 mouse monoclonal antibody.

Immunofluorescence

Anti-IDO1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1.
Anti-IDO1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.

Requested From: 
Date Requested: 5/21/2018

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