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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60065-100 | 100 µg (1.6 mg/ml) | $567 |
![IDN3 / NIPBL Antibody - Anti-IDN3 Antibody - Western Blot. Western blot of Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kD and ~125 kD corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/idn3-nipbl-antibody-ls-c60065/62767_49233.jpg)
Polyclonal Goat anti‑Human IDN3 / NIPBL Antibody (WB) LS‑C60065
Polyclonal Goat anti‑Human IDN3 / NIPBL Antibody (WB) LS‑C60065
Antibody:
IDN3 / NIPBL Goat anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IDN3 / NIPBL Goat anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
NIPBL antibody LS-C60065 is an unconjugated goat polyclonal antibody to human NIPBL (IDN3). Validated for ELISA and WB.
Target
Human IDN3 / NIPBL
Synonyms
NIPBL | CDLS1 | CDLS | Delangin | IDN3 | Nipped-B homolog (Drosophila) | IDN3-B | Scc2 | SCC2 homolog | Nipped-B-like protein
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Orangutan, Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-365 of Human IDN3.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, rat, mouse, dog, bovine and orangutan based on 100% homology for the immunogen sequence. Expect reactivity with multiple splice variants of this protein. Cross reactivity with IDN3 homologues from other sources has not been determined.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:2000 - 1:10000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kD in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weights of 304 kD and 122 kD.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IDN3 / NIPBL
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse
Applications:
ICC, Western blot, Immunoprecipitation
Reactivity:
Monkey
Range:
0.78-50 ng/ml
Species:
Mouse, Human, Rat, Bovine
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.78-50 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
