Target
Human IDH3B
Synonyms
IDH3B | H-IDHB | RP46 | NAD+-specific ICDH
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TTDFIKSVIGHLQTK, from the C-Terminus of protein sequence according to NP_008830.2NP_777281.1.
Specificity
Human IDH3B. This antibody is expected to recognize isoform a (NP_008830.2) and isoform c (NP_777281.1).
Applications
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 40kD band observed in lysates of Mouse fetal Kidney and fetal Skeletal Muscle and of cell line NIH3T3 (calculated MW of 42.2kD according to Human NP_008830.2 and Mouse NP_570954.1). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee