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Antibodies > IDH2 > LS-C407815
 
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Catalog Number Size Price
LS-C407815-10 10 µg $318 
LS-C407815-100 100 µg $470 
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of Hep1-6 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IDH2 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
IDH2 Antibody - IDH2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IDH2 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
IDH2 Antibody - ICC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IDH2 antibody Western blot. All lanes: Anti IDH2 at 0.5 ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Lane 4: SW620 Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: MCF-7 Whole Cell Lysate at 40 ug. Lane 7: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 51 kD. Observed band size: 51 kD.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of Hep1-6 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IDH2 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
IDH2 Antibody - IDH2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
IDH2 Antibody - IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IDH2 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
IDH2 Antibody - ICC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IDH2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IDH2 Antibody - IDH2 antibody Western blot. All lanes: Anti IDH2 at 0.5 ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Lane 4: SW620 Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: MCF-7 Whole Cell Lysate at 40 ug. Lane 7: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 51 kD. Observed band size: 51 kD.
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Polyclonal Rabbit anti‑Human IDH2 Antibody (aa413‑447, IHC, WB) LS‑C407815

Polyclonal Rabbit anti‑Human IDH2 Antibody (aa413‑447, IHC, WB) LS‑C407815

Submit Review LSBio Guarantee
Antibody:
IDH2 Rabbit anti-Human Polyclonal (aa413-447) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407815-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IDH2 Rabbit anti-Human Polyclonal (aa413-447) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Hamster, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
IDH2 antibody LS-C407815 is an unconjugated rabbit polyclonal antibody to IDH2 (aa413-447) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human IDH2
Synonyms
IDH2 | D2HGA2 | ICD-M | IDP | IDPM | MNADP-IDH | NADP(+)-specific ICDH | IDH | IDHM | Isocitrate dehydrogenase | Oxalosuccinate decarboxylase
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human IDH2 (413-447 aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR), identical to the related mouse and rat sequences.
Epitope
aa413-447
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IDH2
P48735 NM_002168 NP_002159.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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