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Anti-IDH / Isocitrate Dehydrogenase Antibody IHC-plus™ LS-B2329

Note: This antibody replaces LS-C41660

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IDH / Isocitrate Dehydrogenase Antibodies

Anti-IDH / Isocitrate Dehydrogenase Antibody IHC-plus™ LS-B2329
Rabbit Polyclonal (IgG) to Pig IDH / Isocitrate Dehydrogenase
Pig, Human, Broad
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IDH / Isocitrate Dehydrogenase Antibody LS-C185091
Rabbit Polyclonal (IgG) to Pig IDH / Isocitrate Dehydrogenase
Pig
Western blot, ELISA, Immunodiffusion, Immunoelectrophoresis
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Pig IDH / Isocitrate Dehydrogenase
Pig, Human, Broad
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Pig IDH / Isocitrate Dehydrogenase
Rabbit
Pig, Human, Broad (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA (1:400 - 1:1600)

Specificity and Use

Purified isocitrate dehydrogenase from porcine heart.
Binds both Dehydroepiandrosterone (DHEA) and DHEAS, demonstrating minimal cross-reactivity with Progesterone and Testosterone. DHEA is the most abundant hormone in the human body and a precursor of all the sex hormones. It is synthesized principally in the adrenal glands and gonads from cholesterol, through conversion to pregnenolone and 17a-Hydroxypregnenolone, by the action of P450 enzymes. DHEA is mainly found in serum as the sulphated form (DHEAS), a reversible reaction catalyzed by sulfotransferase in the adrenal glands, liver and small intestine.
Immunohistochemistry: LS-B2329 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2329 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.2, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.
For research use only.

About IDH / Isocitrate Dehydrogenase

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic.

Immunohistochemistry

Immunohistochemistry
Anti-IDH antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2329 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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