Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
ID2 Antibody, BHLHb26 Antibody, Cell growth-inhibiting gene 8 Antibody, ID2H Antibody, Inhibitor of differentiation 2 Antibody, GIG8 Antibody, Helix-loop-helix protein ID2 Antibody, ID2A Antibody, Inhibitor of DNA binding 2 Antibody
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation.
Western Blot analysis of ID2 expression in transfected 293T cell line by ID2 monoclonal antibody (M01), clone 3C3.Lane 1: ID2 transfected lysate(14.9 KDa).Lane 2: Non-transfected lysate.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged ID2 is approximately 0.3 ng/ml as a capture antibody.
Western blot analysis of ID2 over-expressed 293 cell line, cotransfected with ID2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ID2 monoclonal antibody (M01), clone 3C3 . GAPDH ( 36.1 kDa ) used as specificity and loading control.