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ICOS / CD278 Antibody (clone 7E.17G9) LS‑C107416
CD278 antibody LS-C107416 is an unconjugated rat monoclonal antibody to mouse CD278 (ICOS). Validated for Flow.
Catalog
Size
Price
LS-C107416-50
50 µg
Unavailable
LS-C107416-500
500 µg
Unavailable

Popular ICOS / CD278 Products

Surface staining of CD3 + CD28 stimulated human PBMC with anti-human ICOS (ISA-3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Staining of 3-day unstimulated (left) and 3-day anti-CD3/CD28 stimulated (right) human peripheral blood cells with PE Mouse IgG1, K Isotype Control (blue histogram) or PE anti-human ICOS (purple histogram). Cell in the lymphocytes gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Anti-ICOS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4395 concentration 3.75 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Flow cytometry of ICOS antibody
Species: Human
Applications: Flow Cytometry, ELISA, Functional Assay
Flow cytometry of ICOS antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA, Functional Assay

Product Description

CD278 antibody LS-C107416 is an unconjugated rat monoclonal antibody to mouse CD278 (ICOS). Validated for Flow.
About ICOS / CD278
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Q9Y6W8 AJ277832 CAC06612.1

ICOS Antibody, AILIM Antibody, CD278 Antibody, CVID1 Antibody, Inducible costimulator Antibody, Inducible T-cell co-stimulator Antibody, CD278 antigen Antibody, Inducible T-cell costimulator Antibody


Specifications

Target
Mouse ICOS / CD278
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [7E.17G9]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
ICOS / CD278 antibody was raised against mouse ICOS
Usage
The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and ConA activated (3 day) mouse splenocyte suspensions and can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Flow Cytometry

Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of 3 day ConA activated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.

Flow Cytometry

Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.
Staining of unstimulated BALB/c splenocytes with 0.06 ug of purified rat IgG2b isotype control (open histogram) or 0.06 ug of purified 7E.17G9 (colored histogram) followed by biotin anti-rat IgG and SAv-PE. Total viable cells were used for analysis.


Requested From: 
Date Requested: 6/25/2018

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