Monoclonal Mouse anti‑Human ICAM3 / CD50 Antibody (clone SPM505, Azide‑free,BSA‑free, IHC, IF) LS‑C390377
Monoclonal Mouse anti‑Human ICAM3 / CD50 Antibody (clone SPM505, Azide‑free,BSA‑free, IHC, IF) LS‑C390377
Antibody:
ICAM3 / CD50 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM505) Antibody
Application:
IHC, IHC-P, IF, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
ICAM3 / CD50 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM505) Antibody
Application:
IHC, IHC-P, IF, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
CD50 antibody LS-C390377 is an unconjugated mouse monoclonal antibody to human CD50 (ICAM3). Validated for Flow, IF and IHC.
Target
Human ICAM3 / CD50
Synonyms
ICAM3 | CD50 | CDW50 | ICAM-R | CD50 antigen | ICAM-3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified.
Immunogen
Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.
Specificity
Human ICAM3 / CD50
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues. Optimal dilution of the anti-ICAM-3 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Frozen, Flow Cytometry, ELISA, Functional Assay, Neutralization
Species:
Human
Applications:
Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse
Applications:
Western blot
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Reactivity:
Mouse
Range:
0.78-50 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/28/2024