Human ICAM-1 / CD54

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin.

Immunoaffinity purified

Unmodified

CHO cell derived recombinant Human ICAM-1.

Human ICAM-1

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal adenocarcinoma (moderately differentiated). The recommended concentration is 0.25 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human ICAM-1 (10 ng/ml), a concentration of 8 u/ml of this antibody is required. ELISA: To detect Human ICAM-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ICAM-1 (LS-C104787) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Western Blot: To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05362 NCBI: NM_000201 NP_000192.2