Human ICAM-1 / CD54

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

F4-31C2

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Human umbilical cord vein endothelial cells (HUVEC) were used as the immunogen for the ICAM1 antibody.

Human ICAM-1 / CD54

• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM1 also binds to CD43 and to Plasmodium falciparum infected RBCs and may also be related to progression and metastasis of tumors. Optimal dilution of the ICAM1 antibody should be determined by the researcher.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05362 NCBI: NM_000201 NP_000192.2