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Anti-ICAM-1 / CD54 Antibody (Biotin) LS-C104787

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ICAM-1 / CD54 Antibodies

Anti-ICAM-1 / CD54 Antibody (clone 15.2) IHC-plus™ LS-B3431
Mouse Monoclonal [clone 15.2] (IgG1) to Human ICAM-1 / CD54
Human, Pig
IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-ICAM-1 / CD54 Antibody (Biotin) LS-C123071
Rabbit Polyclonal to Human ICAM-1 / CD54
Human, Baboon, Monkey, Cynomolgus
IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Biotin Conjugated
Anti-ICAM-1 / CD54 Antibody (clone 15.2, Azide-free) LS-C134492
Mouse Monoclonal [clone 15.2] (IgG1,k) to Human ICAM-1 / CD54
Human
Western blot, Flow Cytometry, ELISA
Unconjugated, Azide-free
Flow Cytometry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ICAM-1 / CD54
Human
IHC, Western blot, ELISA (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human ICAM-1 / CD54
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ICAM-1 / CD54 antibody was raised against e.coli derived recombinant Human ICAM-1.
Human ICAM-1
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human ICAM-1 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. To detect Human ICAM-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human ICAM-1 (LS-C104711) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Western Blot: To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About ICAM-1 / CD54

P05362 NM_000201 NP_000192.2

ICAM1 Antibody, BB2 Antibody, CD54 antigen Antibody, CD54 Antibody, ICAM-1 Antibody, p3.58 Antibody

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Immunohistochemistry

Immunohistochemistry
IHC of ICAM-1 / CD54 antibody LS-C104711. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of ICAM-1 / CD54 antibody LS-C104711. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of ICAM-1 / CD54 antibody LS-C104711. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of ICAM-1 / CD54 antibody LS-C104711. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of ICAM-1 / CD54 antibody LS-C104711. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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