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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HYOU1 / ORP150 Antibody (clone 6F7) IHC‑plus™ LS‑B5445
Note: This antibody replaces LS-C133651
ORP150 antibody LS-B5445 is an unconjugated mouse monoclonal antibody to human ORP150 (HYOU1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5445-50
50 µg (0.5 mg/ml)
Unavailable

Popular HYOU1 / ORP150 Products

Anti-HYOU1 / ORP150 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3175 concentration 5 ug/ml.
Species: Rat, Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6661 dilution 1:50.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Western blot of recombinant HYOU1 / ORP150.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, Western blot

Product Description

ORP150 antibody LS-B5445 is an unconjugated mouse monoclonal antibody to human ORP150 (HYOU1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About HYOU1 / ORP150
HYOU1 / ORP150 belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Q9Y4L1 NM_006389 NP_006380.1

HYOU1 Antibody, GRP-170 Antibody, Grp170 Antibody, Glucose-regulated protein 170 Antibody, Hypoxia up-regulated 1 Antibody, Hypoxia up-regulated protein 1 Antibody, HSP12A Antibody, ORP150 Antibody, ORP-150 Antibody


Specifications

Target
Human HYOU1 / ORP150
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [6F7]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HYOU1 / ORP150 antibody was raised against hYOU1 (NP_006380, 901 a.a. ~ 1000 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human HYOU1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4. Sourced in Ascites.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.
Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.

Western blot

Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.
Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.
Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.

Western blot

Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.
Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.
Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.

Western blot

Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.
Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.
Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5445 concentration 5 ug/ml.

Western blot

Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.
Western blot of HYOU1 expression in MCF-7 with HYOU1 monoclonal antibody clone 6F7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HYOU1 is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/18/2018

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