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Anti-HYOU1 / ORP150 Antibody (C-Terminus) IHC-plus™ LS-B3175

Note: This antibody replaces LS-C46842

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HYOU1 / ORP150 Antibodies

Anti-HYOU1 / ORP150 Antibody (clone 6F7) IHC-plus™ LS-B5445
Mouse Monoclonal [clone 6F7] (IgG1,k) to Human HYOU1 / ORP150
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HYOU1 / ORP150 Antibody (Internal, clone EPR5890) IHC-plus™ LS-B6661
Rabbit Monoclonal [clone EPR5890] to Human HYOU1 / ORP150
Human, Mouse, Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-HYOU1 / ORP150 Antibody (aa695-994, Biotin) LS-C299593
Rabbit Polyclonal (IgG) to Human HYOU1 / ORP150
Human
Western blot, ELISA
Biotin Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Rat HYOU1 / ORP150
Rat, Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Rat HYOU1 / ORP150
Rabbit
Rat, Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA

Specificity and Use

HYOU1 / ORP150 antibody was raised against synthetic peptide from rat HYOU1 / ORP150.
C-Terminus
Synthetic peptide corresponding to C-terminal residues of rat Hyou1(Hypoxia up-regulated 1) or Orp150 (150 kD oxygen-regulated protein precursor).
Immunohistochemistry: LS-B3175 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3175 was determined to be 5 ug/ml.

Packaging

PBS, 0.01% sodium azide, 50% glycerol.
Store at -20°C.
For research use only.

Publications (1)

1
A thrombospondin-dependent pathway for a protective ER stress response. Lynch JM, Maillet M, Vanhoutte D, Schloemer A, Sargent MA, Blair NS, Lynch KA, Okada T, Aronow BJ, Osinska H, Prywes R, Lorenz JN, Mori K, Lawler J, Robbins J, Molkentin JD. Cell. 2012 Jun;149:1257-68. [Full Text Article] [PubMed:22682248] [PMC:PMC3372931]

About HYOU1 / ORP150

Q9Y4L1 NM_006389 NP_006380.1

HYOU1 Antibody, GRP-170 Antibody, Grp170 Antibody, Glucose-regulated protein 170 Antibody, Hypoxia up-regulated 1 Antibody, Hypoxia up-regulated protein 1 Antibody, HSP12A Antibody, ORP150 Antibody, ORP-150 Antibody

HYOU1 / ORP150 belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER.

Immunohistochemistry

Immunohistochemistry
Anti-HYOU1 / ORP150 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3175 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HYOU1 / ORP150 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3175 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HYOU1 / ORP150 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3175 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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