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Anti-HYOU1 / ORP150 Antibody (aa274-303) LS-C100905

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular HYOU1 / ORP150 Antibodies

Anti-HYOU1 / ORP150 Antibody (C-Terminus) IHC-plus™ LS-B3175
Rabbit Polyclonal to Rat HYOU1 / ORP150
Rat, Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HYOU1 / ORP150 Antibody (aa274-303) LS-C100905
Rabbit Polyclonal to Human HYOU1 / ORP150
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HYOU1 / ORP150 Antibody (clone 6F7) IHC-plus™ LS-B5445
Mouse Monoclonal [clone 6F7] (IgG1,k) to Human HYOU1 / ORP150
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HYOU1 / ORP150
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human HYOU1 / ORP150
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E8857 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100905. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa274-303
This HYOU1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human HYOU1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HYOU1 / ORP150

Q9Y4L1 NM_006389 NP_006380.1

HYOU1 Antibody, GRP-170 Antibody, Grp170 Antibody, Glucose-regulated protein 170 Antibody, Hypoxia up-regulated 1 Antibody, Hypoxia up-regulated protein 1 Antibody, HSP12A Antibody, ORP150 Antibody, ORP-150 Antibody

HYOU1 / ORP150 belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of HYOU1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of lysate from HepG2 cell line, using HYOU1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
 

Requested From: 
Date Requested: 3/25/2017

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