LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-HYAL1 Antibody (aa36-85) IHC-plus™ LS-B3995

Note: This antibody replaces LS-C102370
Catalog Size Price
LS-B3995-50 50 µg (1 mg/ml) Unavailable

Related Products

42 HYAL1 Antibodies

Most Popular HYAL1 Antibodies

Anti-HYAL1 Antibody (aa36-85) IHC-plus™ LS-B3995
Rabbit Polyclonal (IgG) to Human HYAL1
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HYAL1 Antibody (aa279-309) LS-C322129
Rabbit Polyclonal (IgG) to Human HYAL1
Human
IHC, Western blot, ELISA
Unconjugated
Anti-HYAL1 Antibody (aa32-47) LS-C343927
Rabbit Polyclonal (IgG) to Human HYAL1
Human, Orangutan, Gibbon, Bat
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HYAL1 Antibody LS-C415476
Rabbit Polyclonal to Human HYAL1
Human
IHC, Western blot, ELISA
Unconjugated
Western blot Image
Anti-HYAL1 Antibody (aa72-121, HRP) LS-C454263
Rabbit Polyclonal to Human HYAL1
Human, Chimpanzee, Gibbon, Monkey, Bovine, Dog, Guinea pig, Pig
Western blot
HRP Conjugated
Western blot Image

100% Guaranteed
Rabbit Polyclonal (IgG) to Human HYAL1
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human HYAL1
Rabbit
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Monkey, Pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

HYAL1 antibody was raised against synthetic peptide located between aa36-85 of human HYAL1 (Q12794, NP_695014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Elephant, Bat, Horse, Guinea pig (100%); Galago, Marmoset, Rabbit, Pig (92%); Monkey, Dog (85%); Bovine (83%).
LS-E18349 - Lyophilized - 100 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-B3995. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa36-85
Human HYAL1
Immunohistochemistry: LS-B3995 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3995 was determined to be 10 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HYAL1

Q12794 NM_007312 NP_009296.1

HYAL1 Antibody, FUS2 Antibody, Hyaluronidase-1 Antibody, Hyaluronoglucosaminidase 1 Antibody, LUCA1 Antibody, Hyaluronoglucosaminidase-1 Antibody, Plasma hyaluronidase Antibody, LuCa-1 Antibody, Tumor suppressor LUCA-1 Antibody, HYAL-1 Antibody, Lung carcinoma protein 1 Antibody

HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.

Immunohistochemistry

Immunohistochemistry
Anti-HYAL1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3995 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western analysis of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/22/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number