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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HUWE1 / ARFBP1 Antibody IHC-plus™ LS-B1359

Best Seller
Note: This antibody replaces LS-C34700
ARFBP1 antibody LS-B1359 is an unconjugated rabbit polyclonal antibody to ARFBP1 (HUWE1) from human, mouse and rat. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Catalog
Size
Price
LS-B1359-0.05
0.05 mg (1 mg/ml)
$425
Description
ARFBP1 antibody LS-B1359 is an unconjugated rabbit polyclonal antibody to ARFBP1 (HUWE1) from human, mouse and rat. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human HUWE1 / ARFBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HUWE1 / ARFBP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1. The immunogen is located within amino acids 4250 - 4300 of ARF-BP1.
Blocking Peptide
LS-E30348 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B1359. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About HUWE1 / ARFBP1
Q7Z6Z7 NM_031407 NP_113584.3

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Publications (3)

1
Obesity promotes liver carcinogenesis via Mcl-1 stabilization independent of IL-6R alpha signaling. Gruber S, Straub BK, Ackermann PJ, Wunderlich CM, Mauer J, Seeger JM, B[Character fc]ning H, Heukamp L, Kashkar H, Schirmacher P, Br[Character fc]ning JC, Wunderlich FT. Cell reports. 2013 Aug;4:669-80. [Full Text Article] [PubMed:23954788]
2
Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells. He GQ, Xu WM, Li JF, Li SS, Liu B, Tan XD, Li CQ. Molecular brain. 2015 8:88. (WB, IP, IF; Rat) [Full Text Article] [PubMed:26698301] [PMC:PMC4690333]
3
HUWE1 plays important role in mouse preimplantation embryo development and the dysregulation is associated with poor embryo development in humans. Chen LJ, Xu WM, Yang M, Wang K, Chen Y, Huang XJ, Ma QH. Scientific reports. 2016 6:37928. (WB, IF; Mouse) [Full Text Article] [PubMed:27901130] [PMC:PMC5128802]

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Images

Immunohistochemistry

Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.

Immunofluorescence

Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.

Western blot

Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.
Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.

Immunofluorescence

Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.

Western blot

Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.
Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.

Immunofluorescence

Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.

Western blot

Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.
Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.

Immunofluorescence

Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.

Western blot

Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.
Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.

Requested From: United States
Date Requested: 2/18/2019
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