Human HUS1B

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Mouse

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human Hus1B protein.

A BLAST analysis indicates 100% homology of the immunizing sequence with Hus1B protein from human. Partial reactivity with mouse Hus1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:20000 - 1:100000)

Immunohistochemistry: LS-B376 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B376 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8NHY5 NCBI: NP_683762.2