(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
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Humanin antibody was raised against residues 9-24 (LLLLTSEIDLPVKRRA) of the human Humanin protein. Percent identity by BLAST analysis: Human (100%).
Immunohistochemistry: LS-B237 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B237 was determined to be 5 mg/ml.
PBS, 0.02% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.