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Anti-Human IgA Secondary Antibody (HRP) LS-C134875

Ordering

Wt. Vol. Conc. Price
1200 µg - 1.2 mg/ml $235
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular Human IgA Antibodies

Goat Anti-Human IgA Secondary Antibody (RPE) LS-C56020
Goat Polyclonal to Human IgA
Human
Flow Cytometry
RPE Conjugated
Mouse Anti-Human IgA Secondary Antibody (clone AD3) LS-B3728
Mouse Monoclonal [clone AD3] (IgG1) to Human IgA
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Mouse Anti-Human IgA Secondary Antibody (clone BFG-267, HRP) LS-C202363
Mouse Monoclonal [clone BFG-267] (IgG1) to Human IgA
Human
IHC - Frozen, ELISA, Dot Blot
HRP Conjugated

100% Guaranteed 100% Guaranteed
Human Polyclonal to Human IgA
Human
ELISA
HRP Conjugated

Details

Human IgA
Human
Human (tested or 100% immunogen sequence identity)
Polyclonal
Horseradish Peroxidase
Purified
Unmodified

Applications

ELISA (1:1000)

Specificity and Use

Human IgA antibody was raised against human IgA.
Antibodies were raised in rabbit to human IgA. From the crude polyclonal the cross-reactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound. The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate-te. No cross-reaction with human IgG and IgM in ELISA. No cross-reaction with albumin fraction of human serum. No cross-reaction with albumin fraction of other animal species.
Recommended dilution as secondary antibody in ELISA, 1:1000. Recommended dilution as secondary antibody in dot-blotting, 1:100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50% glycerol, 0.1 M sodium chloride, 0.01 M sodium phosphate at pH 7.5
Store at 4°C
For research use only.

About Human IgA

Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in mucosal immunity. More IgA is produced in mucosal linings than all other types of antibody combined; between three and five grams are secreted into the intestinal lumen each day. This accumulates up to 15% of the total immunoglobulin produced in the entire body. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA).

Requested From: United States
Date Requested: 12/3/2016

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