Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
Human IgG, IgM and IgA.
Antibodies were raised in goat to human IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound human immuno-globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human Immunoglobulins a minimum of 65% protein bound. The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. No cross-reaction with albumin fraction of human serum other then IgG, IgM and IgA. No cross-reaction with albumin fraction of other animal species.
Recommended dilution as secondary antibody in ELISA, 1:1000. Recommended dilution as secondary antibody in dot-blotting, 1:100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
50% glycerol, 0.1 M sodium chloride, 0.0 1 M sodium phosphate at, pH 7.5