Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
Human IgG, IgM, and IgA.
Antibodies were raised in rabbit to human IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound human immuno- globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human immunoglobulins a minimum of 65% protein bound. The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of rabbit IgG. Low background fluorescence of normal tissue in contrast to autoimmune disease tissues with deposited immunoglobulins. No cross reactivity with albumin fractions of human serum. No cross reactivity with albumin fractions of animal species.
For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
50% glycerol, 0.1 M sodium chloride, 0.0 1 M sodium phosphate at, pH 7.5