Human IgA + IgG + IgM
(tested or 100% immunogen sequence identity)
Human IgG, IgM and IgA.
Antibodies were raised in goat to human IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound human immuno-globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human Immunoglobulins a minimum of 65% protein bound. The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. No cross-reaction with albumin fraction of human serum other then IgG, IgM and IgA. No cross-reaction with albumin fraction of other animal species.
Recommended dilution as secondary antibody in ELISA, 1:1000. Recommended dilution as secondary antibody in dot-blotting, 1:100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
50% glycerol, 0.1 M sodium chloride, 0.0 1 M sodium phosphate at, pH 7.5
For research use only.
This antibody carries the LSBio 100% Guarantee.