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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2043-50 | 50 µg (0.5 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human HTRA3 Antibody (aa250‑300, IHC, WB) LS‑B2043
IHC‑plus™ Polyclonal Rabbit anti‑Human HTRA3 Antibody (aa250‑300, IHC, WB) LS‑B2043
Note: This antibody replaces LS-C699
Antibody:
HTRA3 Rabbit anti-Human Polyclonal (aa250-300) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HTRA3 Rabbit anti-Human Polyclonal (aa250-300) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
HTRA3 antibody LS-B2043 is an unconjugated rabbit polyclonal antibody to HTRA3 (aa250-300) from human. It is reactive with human, rat and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human HTRA3
Synonyms
HTRA3 | HtrA serine peptidase 3 | Serine protease HTRA3 | Tasp | Probable serine protease HTRA3 | PRSP
Host
Rabbit
Reactivity
Human, Monkey, Rat
(tested or 100% immunogen sequence identity)
Predicted
Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Amino acids 273-287 (IVSTAQREGRELGLR)of human HtrA3 were used as immunogen. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%); Gorilla, Bovine, Pig, Pufferfish (93%); Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%); Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).
Epitope
aa250-300
Specificity
The amino acid sequence is 100% homologous in HtrA3, 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 3 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2043 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2043 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HTRA3
Validation
Anti-HTRA3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HTRA3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HTRA3 Antibody (aa250-300) for IHC, WB/Western LS-B2043 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
The high-temperature requirement factor A3 (HtrA3) is associated with acquisition of the invasive phenotype in oral squamous cell carcinoma cells. Moriya Y, Uzawa N, Morita T, Mogushi K, Miyaguchi K, Takahashi K, Michikawa C, Sumino J, Tanaka H, Harada K. Oral oncology. Jan;51:84-9.
Customer Reviews (0)
Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
Porcine
Range:
46.875-3000 pg/ml
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Reactivity:
Human
Range:
15.6-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
