Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
HTR3A / 5-HT3A Receptor belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation.
Western blot of lysates using 1 ug/ml Rabbit Anti-5HT3A Polyclonal Antibody. Lane 1, 3: HeLa cell lysate. Lane 2, 4: Mouse brain tissue lysate. Primary Antibody: Lane 1, 2: Rabbit Anti-5HT3A Polyclonal Antibody. Lane 3, 4: Rabbit Anti-5HT3A Polyclonal Antibody pre-incubated with immunizing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Flow cytometry of HepG2 cells using 5HT3A Antibody, pAb, Rabbit (shaded histogram) or with an isotype. Control antibody (open histogram), followed by R-PE conjugated anti-rabbit IgG.