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Anti-HTR3A / 5-HT3A Receptor Antibody (aa21-36) IHC-plus™ LS-B2042

Note: This antibody replaces LS-C795, LS-C75763

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HTR3A / 5-HT3A Receptor Antibodies

Anti-HTR3A / 5-HT3A Receptor Antibody (N-Terminus) IHC-plus™ LS-B152
Rabbit Polyclonal to Human HTR3A / 5-HT3A Receptor
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HTR3A / 5-HT3A Receptor Antibody (aa21-36) IHC-plus™ LS-B2042
Rabbit Polyclonal (IgG) to Rat HTR3A / 5-HT3A Receptor
Rat, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HTR3A / 5-HT3A Receptor Antibody (aa428-439) IHC-plus™ LS-B3945
Goat Polyclonal to Human HTR3A / 5-HT3A Receptor
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Rat HTR3A / 5-HT3A Receptor
Rat, Human
IHC - Paraffin, Western blot
Unconjugated

Details

Rat HTR3A / 5-HT3A Receptor
Rabbit
Rat, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

HTR3A / 5-HT3A Receptor antibody was raised against this antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949. Percent identity by BLAST analysis: Rat (100%); Mouse (88%); Hamster (81%).
aa21-36
In human brain, a 47-50 kD band is observed.
Immunohistochemistry: LS-B2042 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2042 was determined to be 5 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HTR3A / 5-HT3A Receptor

P46098 NM_000869 NP_000860.2

HTR3A Antibody, 5-HT3-A Antibody, 5-HT3R-A Antibody, 5-HT3 receptor Antibody, 5HT3 serotonin receptor Antibody, 5-HT3A Antibody, 5-HT3a receptor Antibody, 5-hydroxytryptamine receptor 3 Antibody, 5-HT-3 Antibody, 5-HT3 Antibody, 5-HT3R Antibody, 5HT3R Antibody, HTR3 Antibody, Serotonin receptor 3A Antibody

HTR3A / 5-HT3A Receptor belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation.

Immunohistochemistry

Immunohistochemistry
Anti-5HT3A receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2042 concentration 5 ug/ml.

Western blot

Western blot
Western blot of 5-HT3R in human brain tissue lysate with anti-5-HT3R pcAb . A protein band of approximate molecular weight of 47-50 kDa was detected.

Requested From: United States
Date Requested: 12/3/2016

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