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Anti-HT2A / TRIM32 Antibody (aa549-598) IHC-plus™ LS-B1105

Note: This antibody replaces LS-C30308

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HT2A / TRIM32 Antibodies

Anti-HT2A / TRIM32 Antibody (aa549-598) IHC-plus™ LS-B1105
Rabbit Polyclonal to Human HT2A / TRIM32
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HT2A / TRIM32 Antibody (aa169-198) IHC-plus™ LS-B14406
Rabbit Polyclonal to Human HT2A / TRIM32
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human HT2A / TRIM32
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human HT2A / TRIM32
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

HT2A / TRIM32 antibody was raised against synthetic peptide located between aa549-598 of human TRIM32 (Q13049, NP_036342). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%); Stickleback, Zebrafish (84%).
LS-E11077 - Lyophilized - 100 µg - $145.00
aa549-598
Human TRIM32
Immunohistochemistry: LS-B1105 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1105 was determined to be 2.5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Kudryashova E, Wu J, Havton LA, Spencer MJ. Human molecular genetics. 2009 18:1353-67. (WB; Mouse) [Full Text Article] [PubMed:19155210] [PMC:PMC2722196]

About HT2A / TRIM32

Q13049 NM_012210 NP_036342.2

TRIM32 Antibody, 72 kDa Tat-interacting protein Antibody, HT2A Antibody, LGMD2H Antibody, TATIP Antibody, Tripartite motif containing 32 Antibody, Zinc finger protein HT2A Antibody, Zinc-finger protein HT2A Antibody, TAT-interactive protein, 72-KD Antibody, BBS11 Antibody, Tripartite motif-containing 32 Antibody

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

Immunohistochemistry

Immunohistochemistry
Anti-TRIM32 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1105 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TRIM32 antibody LS-B1105 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Transfected 293T Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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