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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPG2 / Perlecan Antibody (clone A7L6) LS‑C15761
Antibody
Perlecan antibody LS-C15761 is an unconjugated rat monoclonal antibody to Perlecan (HSPG2) from human, mouse, bovine and other species. Validated for IF and IHC.
Catalog
Size
Price
LS-C15761-500
500 µl (0.2 mg/ml)
Unavailable

Popular HSPG2 / Perlecan Products

Antibody
Species: Bovine, Human
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-HSPG2 / Perlecan antibody IHC of human thymus, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2468 concentration 5 ug/ml.
Species: Bovine, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
IHC with HSPG2 antibody on frozen human spleen.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Frozen, ICC, Western blot, Immunoprecipitation

Product Description

Perlecan antibody LS-C15761 is an unconjugated rat monoclonal antibody to Perlecan (HSPG2) from human, mouse, bovine and other species. Validated for IF and IHC.
About HSPG2 / Perlecan
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. P98160 NM_005529 NP_005520.4

HSPG2 Antibody, Endorepellin (domain V region) Antibody, HSPG Antibody, Heparan sulfate proteoglycan 2 Antibody, Perlecan Antibody, Perlecan proteoglycan Antibody, SJA Antibody, SJS1 Antibody, PRCAN Antibody, SJS Antibody


Specifications

Target
Mouse HSPG2 / Perlecan
Host
Rat
Reactivity
Mouse, Human, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [A7L6]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (1 - 2 µg/ml)
  • Immunofluorescence
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
  • Western blot
Immunogen
Murine EHS laminin preparation.
Specificity
Recognizes perlecan. Does not cross-react with laminin, fibronectin or dermatran sulfate proteoglycan. Species cross-reactivity: Human, bovine, pig, mouse.
Usage
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Frozen and acid-alcohol fixed): 1-2 ug/ml for 30 minutes at RT. Staining of acid-alcohol-fixed tissues requires antigen unmasking with 15000u/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37?C. Positive control: Tonsil.
Presentation
PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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